|Lipid, lipid rafts and caveolin-1 in regulation of lysosome and autophagy in cancer biology|
报告人：Shen Han-Ming, MD, PhD
Department of Physiology, Yong Loo Lin School of Medicine,
NUS Graduate School for Integrative Sciences and Engineering,
National University of Singapore
In the past several years, we have studied the lipid and lipid rafts in regulation of autophagy. In this presentation, I will discuss the following 3 aspect of our work, including (i) free fatty acid in regulation of autophagy, (ii) critical role of Stearoyl-CoA Desaturase 1 (SCD-1) in lipogenesis and autophagy, and (iii) our ongoing work on lipid rafts and caveolin-1 (Cav-1) in lysosomal function and autophagy. We demonstrate for the first time that Cav-1 deficiency promotes both the basal and inducible autophagy. Interestingly, the promoting effect was found mainly on the late stage of autophagy via enhancing lysosomal function and autophagosome-lysosome fusion. Furthermore, the elevated autophagy level induced by Cav-1 deficiency serves as a cell survival mechanism under starvation. Importantly, down-regulation of Cav-1 and enhanced autophagy level were observed in human breast cancer cells and tissues. Our findings support the notion that autophagy is an important pro-survival mechanism, especially for cells under various stress conditions, and for cancer cells with Cav-1 deficiency. Moreover, our data also suggest that breast cancer patients with low or defective Cav-1 are suitable for autophagy-targeted chemotherapy.
Dr. Shen Han-Ming graduated from Zhejiang Medical University, China, with a Bachelor of Medicine and Master of Medicine in 1985 and 1988, respectively, and a PhD from NUS in 1996. He received his postdoc training in NUS and in the National Cancer Institute, NIH, USA. The main research interest of his lab at the Department of Physiology, NUS is on autophagy and cancer cell biology. Up to date, Dr. Shen has published more than 130 articles in peer-reviewed international journals, with more than 8000 total citations and an H-index at 52. He is the Editorial Board member of Autophagy and Frontiers in Cell and Developmental Biology, and the Academic Editor of PLOS ONE.